11210 生物技術概論

# Required Courses # Undergraduate Program
14 Lectures / 37 Sections / 22h 32m 38s
Lecture 1：Overview of Biotechnology
Section 1
28:45
Section 2
11:27
Lecture 2：Overview of Biological Systems
Section 1
37:04
Section 2
37:37
Section 3
32:49
Section 4
49:42
Section 5
15:16
Lecture 3：Metabolism and Growth
Section 1
38:25
Section 2
22:45
Section 3
50:32
Section 4
47:59
Lecture 4：Genetic Engineering-Procaryote
Section 1
44:05
Section 2
37:16
Section 3
40:39
Section 4
50:36
Section 5
47:55
Section 6
52:32
Section 7
44:09
Section 8
49:01
Section 9
25:45
Lecture 5：Genetic Engineering: Yeast and Filamentous Fungi
Section 1
22:37
Section 2
38:56
Section 3
13:09
Lecture 6：Culture of Animal Cells
Section 1
28:23
Section 2
32:03
Section 3
18:52
Lecture 7：Microbial Process Kinetics
Section 1
26:57
Section 2
53:29
Section 3
49:00
Lecture 8：Bioreactor
Section 1
44:39
Section 2
49:29
Lecture 9：Downstream Process
Section 1
22:34
Section 2
43:44
Section 3
50:53
Section 4
46:37
Section 5
46:57
Section 6
00:00
Lecture 10：Bio-based Production of Chemicals
Lecture 11：Antibiotics
Lecture 12：Amino Acids
Lecture 13：Organic Acids
Lecture 14：Microbial Polyhydroxyalkanoates, Polysaccharides and Lipids
Introduction
Duration｜ 22h 32m 38s
Total Lectures｜ 14 Lectures 37 Sections

Brief course description

This course covers basic cell biology, fundamental genetic engineering, cell culture, bioreactor design, downstream bioprocesses and applications of biotechnology. In the end of the semester, the students are grouped to design and present the production process of some products, starting from market research, genetic engineering of the cells, production process (including bioreactor design and operation) and downstream purification process.

Course keywords

基因工程 (genetic engineering), 細胞培養 (cell culture and fermentation), 下游程序工程(downstream process), 生質化學品 (bio-derived chemicals), 生技產業(biotechnology industry)

Syllabus

Basics:

Chap 1 Overview of Biotechnology
Chap 2 Overview of Biological Systems
Chap 3 Growth and metabolism
Chap 4 Genetic Engineering-Procaryote
Chap 5 Genetic Engineering-Yeast and Fungi
Chap 6 Culture of Animal Cells
Chap 7 Microbial Process Kinetics
Chap 8 Bioreactor
Chap 9 Downstream Process

Applications:

Chap 10 Bio-based Production of Chemicals
Chap 11 Antibiotics
Chap 12 Amino Acids
Chap 13 Organic Acids
Chap 14 Microbial Polyhydroxyalkanoates, Polysaccharides and Lipids

Lectures
Lecture 1：Overview of Biotechnology
Section 1 - Lecture 01
28:45
Section 2 - Lecture 02
11:27
Lecture 2：Overview of Biological Systems
Section 1 - Lecture 02 (Cells, Biomolecules)
37:04
Section 2 - Lecture 03 (Prokaryotes)
37:37
Section 3 - Lecture 04 (Eukaryotes, Cell Components)
32:49
Section 4 - Lecture 05 (Amino Acids, Polypeptide, Lipids)
49:42
Section 5 - Lecture 06 (Carbohydrates)
15:16
Lecture 3：Metabolism and Growth
Section 1 - Lecture 06 (Metabolism, Catabolic Pathways)
38:25
Section 2 - Lecture 07 (Catabolic Pathways)
22:45
Section 3 - Lecture 08 (Catabolic Pathways)
50:32
Section 4 - Lecture 09 (Catabolic Pathways, Anabolic Pathways)
47:59
Lecture 4：Genetic Engineering-Procaryote
Section 1 - Lecture 10 (DNA, DNA Replication)
44:05
Section 2 - Lecture 11 (DNA Replication, RNA, Transcription)
37:16
Section 3 - Lecture 12 (Transcription, Translation)
40:39
Section 4 - Lecture 13 (Translation, Post-Translational Processing, Genetic Engineering)
50:36
Section 5 - Lecture 14 (Genetic Engineering)
47:55
Section 6 - Lecture 15* (Genetic Engineering)
52:32
Section 7 - Lecture 16 (Genetic Engineering, Vectors)
44:09
Section 8 - Lecture 17* (Vectors, Analysis of Gene Expression)
49:01
Section 9 - Lecture 18* (Vectors)
25:45
Lecture 5：Genetic Engineering: Yeast and Filamentous Fungi
Section 1 - Lecture 18* (Introduction, Introdcuing DNA into fungi)
22:37
Section 2 - Lecture 19 (Introdcuing DNA into fungi, Biological applications of fungi)
38:56
Section 3 - Lecture 20 (Biological applications of fungi)
13:09
Lecture 6：Culture of Animal Cells
Section 1 - Lecture 20 (Introduction, Equipment, Culture vessels, Aseptic technique)
28:23
Section 2 - Lecture 21 (Aseptic technique, Medium)
32:03
Section 3 - Lecture 22 (Medium, Preparation and Sterilization)
18:52
Lecture 7：Microbial Process Kinetics
Section 1 - Lecture 22 (Introduction, Kinetic models of cell growth)
26:57
Section 2 - Lecture 23 (Kinetic models of cell growth, Mass balance for ideal bioreactors)
53:29
Section 3 - Lecture 24 (Simulation of the Process)
49:00
Lecture 8：Bioreactor
Section 1 - Lecture 25 (Introduction, Bioreactor configurations)
44:39
Section 2 - Lecture 26 (Bioreactor configurations, Bioreactor design features, Design for sterile operation)
49:29
Lecture 9：Downstream Process
Section 1 - Lecture 27 (Introduction, Solid-liquid separation)
22:34
Section 2 - Lecture 28 (Solid-liquid separation, Release of intracellular products)
43:44
Section 3 - Lecture 29 (Concentration of biological products)
50:53
Section 4 - Lecture 30 (Concentration of biological products)
46:37
Section 5 - Lecture 31 (Purification by Chromatography)
46:57
Section 6 - Lecture 32 (Purification by Chromatography)
Lecture 10：Bio-based Production of Chemicals
Lecture 11：Antibiotics
Lecture 12：Amino Acids
Lecture 13：Organic Acids
Lecture 14：Microbial Polyhydroxyalkanoates, Polysaccharides and Lipids
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Prof. Yu-Chen Hu
Prof. Yu-Chen Hu

Dept. Chemical Engineering

Research Field

Gene Therapy, Tissue Engineering, Development of New Vaccine Platform, CRISPR-based Genome Editing and Regulation, Cancer Therapy

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